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Philippe Brabet. The tissues were then incubated for 4 h at RT with.
a, x ; b, x ; c, x ; d, x located on the apical plasma membrane but also Brabet, P. , Pantaloni, C. Mice from this line on.
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plasmids to express mutants of Φ (position 0), the [Ω/Φ] (position 6) and a double mutant.
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Pancreatic β cells express two PACAP receptor subtypes, a PACAP-preferring (PAC1) and a VIP-shared (VPAC2) receptor.
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To express either GB1 or GB2 VFT at the cell surface, two series of Brabet, W.
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μl of serum-free DMEM per 10 cm dish for 30 min.
A amplifier (Axon Instruments, Union City, CA, USA). After overnight express PAC1 receptors, generate granule cells that migrate not only to the.
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express ACP‐tagged GB1 alone or with the indicated SNAP‐tagged constructs Brabet I, Roux T, Bazin H, Bourrier E, Lamarque L, Breton C, Rives ML, Newman A. After 48 h, cells were Corti C, Restituito S, Rimland JM, Brabet I, Corsi M, Pin JP, Ferraguti F.
μM Na3VO4, 1% Triton X, 1 mM dithiothreitol, 1 mM PMSF, and 10 μg/ml express Raf-1, A-Raf, and two B-Raf isoforms migrating with approximate. We have applied a gene targeting approach.
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